Year: 2014 | Month: March | Volume 7 | Issue 1
Optimization of Culture Condition and Influence of Mediators on Degradation of Reactive Magenta-HB Dye Using Ganoderma cupreum AG-1
Abstract: <div>Reactive dyes are important chemical pollutants from the textile industries. Utilization of microbes including white rot fungi for decolorization of synthetic dyes is one promising strategy of an environmental friendly and cost-competitive alternative to physico-chemical decomposition processes for the treatment of industrial effluents. The present study investigates the</div><div>biodegradation and decolorization of reactive dyes using white rot fungi Ganoderma cupreum AG-1. Physico-chemical parameters including carbon and nitrogen sources, initial glucose and yeast extract concentration, pH, temperature, initial dye concentration, along with the effect of various metal ions and mediators were studied for the decolorization of Reactive Magenta-HB dye. The fungal isolate efficiently decolorized Reactive Magenta HB within 48 h at 28°C, at pH 5.0 and under optimized conditions 94.20</div><div>±1% dye decolorization was obtained within 48 h. The effect of mediators on laccase, manganese peroxidase (MnP) and manganese independent peroxidase (MnIP) were also studied. The decolorization of Reactive Magenta-HB dye was monitored by UV–Visible spectroscopy while dye degradation was studied by HPTLC and FTIR spectroscopy.</div>
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